Effect of the developmental stage and thawing temperature on
the survival and development of the vitrified embryos
Authors:
ElNaggar Mostafa M
Issue Date:
2008
Citation:
Effect of the developmental stage and thawing temperature on
the survival and development of the vitrified embryos Mostafa M ElNaggar [et al]Journal of Taibah University medical sciences مجلة جامعة طيبة العلوم الطبية Taibah UniversityVol 3 no 2 (2008) p p 8391ElNaggar Mostafa M
Abstract:
Objectives
The best developmental stage and the best thawing protocol suitable for cryopreservation of
the early embryos are not well documented The present study aimed at evaluating the effect
of the ultra rapid cryopreservation (vitrification) technique followed by slow or fast thawing
protocol on the fertilized ova 4cell embryos and morula
Methods
The vitrification method included equilibrating the ova in the vitrification solution (EFS40 ;
consisted of 40 % ethylene glycol 30 % Ficoll 05 M sucrose in DPBS) for 2 minutes before
immersion in liquid nitrogen Slow and fast thawing were done and the cryoprotectants were
withdrawn by a hyperosmolar sucrose solution which was then gradually diluted and
replaced by culture medium
Results
The best results were obtained with vitrification of the 4cell embryos both with slow and
fast thawing which gave survival rate of 86% and 94% and in vitro development rate of 74%
and 80% respectively Fast thawing showed better survival rates (80% 94% 77%) and better
in vitro development rates (60% 80% 63%) than those of slow thawing following
vitrification of the fertilized ova 4cell embryos and morula respectively
Conclusion
These criteria of vitrification thawing could be inferred to the human 4cell embryos in the
IVF protocol
