Studies on the biosynthesis of Lserineglutamate transaminase in alternaria chlamydospora

Abstract

Cell free extracts of the filamentous fungus alternaria chlamydospora grown on Lserine as sole source of nitrogen contained Lserineglutamate transaminase (EC 26152) that catalyzes the transamination between Lserine and aketoglutarale to equimolar amounts of pyruvate and glutamate Lserine glutamate transminase was produced during the logarithmic phase of growth maximal growth and enzyme formation were obtained after 3 days of growth The optimum PH for growth and enzyme synthesis was 50 Lserineglutamate transaminase was induced by Lserine Lalanine Lthreonine and Lglutamic acid Sucrose was the best carbon source induced synthesis of enzyme Enzyme activity was repressed by all kind of metal salts The effect of different vitamins on growth and enzyme production was studied